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Seeing is believing!

6/15/2022

 
Our VDK+ Test Kit has a growing user base and has supplanted sniff tests, distillation set-ups, and even chromatography installations. We have a couple case studies in the works, but see below for a sneak peek!

The below image is a real-world photo taken by one of our users. Left: The image on the left is a dry-hopped beer that exhibited hop creep. The beer nearly passed sensory panel analysis before supporting evidence from the VDK+ Test Kit helped stop an early cold crash. Right: The image on the right is from a packaged product that passed forced sensory analysis before our VDK+ Test Kit was implemented at the brewery. Moving forward, the VDK+ Test Kit will help guide ALDC additions to ensure diacetyl concerns are a thing of the past!

Picture

VDK+ Test Kit: What does it mean when you see a dark circle?

6/15/2022

 
First off, make sure you take photos so that we can help troubleshoot! There are some troubleshooting steps we can take to diagnose what is happening. It is possible that this is a real result. 2,3-pentanedione and/or acetoin, which are vicinal diketones (VDK's) similar to 2,3-butanedione (diacetyl) sometimes show up as a dark circle, especially with certain types of yeast. You won't necessarily detect any diacetyl via sensory because 2,3-pentanedione and acetoin are not as potent as diacetyl. However, before we can say that, I want to make sure everything else about the test is running smoothly.
  • Are you writing with Sharpie on the lid/cap? That can bleed into the filter paper.
  • Have you run a water blank? Run the test with 10 mL of distilled water (or even tap water). Do you still see the dark circle?
  • When you see the dark circle, can you detect any diacetyl sensory-wise? That will help lead troubleshooting.
  • Is the height of the water in the water bath above the level of the liquid in the vials?
  • Is the dark circle occurring in real beer samples or samples with controls? If controls, what controls are you using? We have found that controls from Aroxa do not actually contain diacetyl and manifest as that dark circle because they are using one of the other VDKs mentioned above.
  • Have you measured the actual water temp of the water bath? I have seen the dark circle when the water bath is hotter than 70C.

VDK+ Test Kit: Helpful Tips

5/17/2022

 
  • Make sure all chemicals are being stored correctly.  All chemicals are labeled with how they should be stored but here is a quick run down
    • Reagent A - room temp in dark
    • Reagent B - before reagent C is mixed in - room temp 
    • Reagent C - before mixed with reagent B - room temp 
    • Reagent B +C - put in refrigerator 
    • activation capsules - room temp
    • VDK caps - room temp 
  • Make sure all samples are treated the same. You should be filtering out yeast and make sure you are using the same filters. Make sure they are taking the same amount of time to filter. If you are centrifuging yeast out, make sure that is always done for the same amount of time between samples. Make sure samples are all the same temperature when you are running the tests.  For example let any samples what may have been stored in the fridge warm up to room temp before running the assay. 
  • Make sure your hot bath is at 70C. Use an external thermometer to double check any digital read outs.  Make sure that the hot water baths' water  line is above the sample's water line.
  • Only run 1 to 2 tests at a time. 
  • Make sure you are mixing the Reagent A+B+C  mixture well before adding it to the caps. 
  • This is the order we add everything when we run a test:
    • Add Activation Capsule to glass vial
    • Mix Reagent A+B+C
    • Measure out sample and set to side
    • Add Reagent A+B+C to cap.
    • Pour sample in to glass vial, place cap onto vial immediately on top.
    • Place into hot bath for 15 minutes.  
  • Try to keep the order and the time it takes to add everything the same.

Validation of the Beyers Chemical VDK+ Test Kit for Go/No-go Applications

6/4/2021

 
Click the button below to download a white paper written by our very own Emma Kerman as she explains what the results mean from the CSU/Gilded Goat validation performed in Spring 2021.
Download

The Dangers of the Sniff Test

6/4/2021

 
By: Emma Kerman, Beyers Chemical Account Manager
If you’re in the brewing community, there’s a good chance that you’ve heard of, if not performed, a “sniff test”. The sniff test is one of the few methods used to test for VDKs like diacetyl. It’s very common amongst microbreweries and home brewers because it doesn’t require significant time or access to the equipment needed for the standard ASBC methods, and it still provides a quality control measurement to determine the end of fermentation. 

If you aren’t familiar with the sniff test, the basic premise is to take two samples of beer, heat one of them up, and check to see if the unheated sample smells more buttery than the heated one. If the samples smell different, there is still diacetyl and possibly the odorless precursor alpha-acetolactate in the beer and fermentation should continue. If the samples smell the same, you can assume that all of the alpha-acetolactate was converted to diacetyl and got fully metabolized by the yeast, signaling the end of fermentation. 

While this method is quick and essentially free, it is very informal and can be quite unreliable. The olfactory organs can vary so much from person to person, some people can’t even detect diacetyl at all! It can also easily be impacted by something as small as the common cold. In addition, the sniff test can propose physical health risks. There’s a medical condition associated with diacetyl inhalation called popcorn lung, also known as obliterative bronchiolitis (OB). It was first discovered in popcorn factories where workers were constantly exposed to the diacetyl added to enrich the popcorn’s buttery flavor. OB occurs when there is damage to the lungs smallest airways and that damage is irreversible. 

That’s where the Beyers Chemical Co. VDK+ Test Kit comes in. Our proprietary test strips accurately test for VDKs in only 15 minutes, don’t require fancy equipment or lab training, results are comparable to the standard ASBC methods, and don't require any direct inhalation which helps keep brewers safe. Making them a perfect quality control option for both commercial and home brewers!

Resources on Obliterative Bronchiolitis:
1. CDC - Flavorings-Related Lung Disease - NIOSH Workplace Safety and Health Topic. (2017, October 03). Retrieved from https://www.cdc.gov/niosh/topics/flavorings/default.html

2. J.S. Fedan, J.A. Dowdy, K.B. Fedan, A.F. Hubbs, Popcorn worker's lung: In vitro exposure to diacetyl, an ingredient in microwave popcorn butter flavoring, increases reactivity to methacholine, Toxicology and Applied Pharmacology, Volume 215, Issue 1, 2006, Pages 17-22

3. Palmer, Scott M., et al. “Severe Airway Epithelial Injury, Aberrant Repair and Bronchiolitis Obliterans Develops after Diacetyl Instillation in Rats.” PLoS ONE, vol. 6, no. 3, 2011, doi:10.1371/journal.pone.0017644. 

Evaluation of Beyers Chemical VDK+ Test Kit compared to GC and Distillation by independent labs

6/4/2021

 
Students from Colorado State University's Fermentation Science & Technology program teamed up with Gilded Goat Brewing Co. and two independent labs to independently evaluate the Beyers Chemical VDK+ Test Kit. Download the poster below to see what they found out!
Download

Survey of Brix and TA in kombucha

2/17/2021

 
Download the white paper provided below to see how TA and Brix compare in over 50 different kombucha samples provided by producers at KombuchaKon 2019.

Survey of Brix and TA in Kombucha

Download

Quality Control Checkpoints for Beer

2/12/2021

 
Download the poster provided below to see common quality control checkpoints in the beer brewing process.
Download

How to use the VDK+ Test Strips?

2/12/2021

 
Download the white paper below to see how you can use the VDK+ Test Strips to improve your beer quality program!
download

Why spend money on VDK+ Test Strips?

2/12/2021

 
Download the whitepaper below to see why VDK+ Test Strips could save you money!
Download
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